VAT Commands in Exercise

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VAT Commands in Exercise

 
 vtools -h
 vtools init VATDemo
 vtools import *.vcf.gz --var_info DP filter --geno_info DP_geno --build hg18 -j1
 vtools liftover hg19
 head phenotypes.csv
 vtools phenotype --from_file phenotypes.csv --delimiter ","
 vtools show project
 vtools show tables
 vtools show table variant
 vtools show samples
 vtools show genotypes
 vtools show fields
 vtools select variant --count
 vtools show genotypes > GenotypeSummary.txt
 head GenotypeSummary.txt
 vtools output variant "max(DP)" "min(DP)" "avg(DP)" "stdev(DP)" "lower_quartile(DP)" "upper_quartile(DP)" --header
 vtools select variant "filter='PASS'" --count
 vtools select variant "filter='PASS'" -o "max(DP)" "min(DP)" "avg(DP)" "stdev(DP)" "lower_quartile(DP)" "upper_quartile(DP)" --header
 vtools update variant --from_stat 'total=#(GT)' 'num=#(alt)' 'het=#(het)' 'hom=#(hom)' 'other=#(other)' 'minDP=min(DP_geno)' 'maxDP=max(DP_geno)' 'meanDP=avg(DP_geno)' 'maf=maf()'
 vtools show fields
 vtools show table variant
 vtools update variant --from_stat 'totalGD10=#(GT)' 'numGD10=#(alt)' 'hetGD10=#(het)' 'homGD10=#(hom)' 'otherGD10=#(other)' 'mafGD10=maf()' --genotypes "DP_geno > 10"
 vtools show fields
 vtools show table variant
 vtools output variant chr pos maf mafGD10 --header --limit 20
 vtools phenotype --set "RACE=0" --samples "filename like 'YRI%'"
 vtools phenotype --set "RACE=1" --samples "filename like 'CEU%'"
 vtools show samples --limit 10
 vtools update variant --from_stat 'CEU_mafGD10=maf()' --genotypes 'DP_geno>10' --samples "RACE=1"
 vtools update variant --from_stat 'YRI_mafGD10=maf()' --genotypes 'DP_geno>10' --samples "RACE=0"
 vtools output variant chr pos mafGD10 CEU_mafGD10 YRI_mafGD10 --header --limit 10
 vtools phenotype --from_stat 'CEU_totalGD10=#(GT)' 'CEU_numGD10=#(alt)' --genotypes 'DP_geno>10' --samples "RACE=1"
 vtools phenotype --from_stat 'YRI_totalGD10=#(GT)' 'YRI_numGD10=#(alt)' --genotypes 'DP_geno>10' --samples "RACE=0"
 vtools phenotype --output sample_name CEU_totalGD10 CEU_numGD10 YRI_totalGD10 YRI_numGD10 --header
 vtools execute ANNOVAR geneanno
 vtools output variant chr pos ref alt mut_type --limit 20 --header
 vtools_report trans_ratio variant -n num
 vtools_report trans_ratio variant -n numGD10
 vtools select variant "DP<15" -t to_remove
 vtools show tables
 vtools remove variants to_remove -v0
 vtools show tables
 vtools remove genotypes "DP_geno<10" -v0 
 vtools select variant "mut_type like 'non%' or mut_type like 'stop%' or region_type='splicing'" -t v_funct 
 vtools show tables
 vtools show samples --limit 5
 vtools select variant --samples "RACE=1" -t CEU
 mkdir -p ceu
 cd ceu
 vtools init ceu --parent ../ --variants CEU --samples "RACE=1" --build hg19
 vtools show project
 vtools select variant "CEU_mafGD10>=0.05" -t common_ceu
 vtools select v_funct "CEU_mafGD10<0.01" -t rare_ceu 
 vtools use refGene 
 vtools show annotation refGene
 vtools associate -h
 vtools show tests
 vtools show test LinRegBurden
 vtools associate common_ceu BMI --covariate SEX -m "LinRegBurden --alternative 2" -j1 --to_db EA_CV > EA_CV.asso.res
 grep -i error *.log
 less EA_CV.asso.res
 sort -g -k7 EA_CV.asso.res | head
 vtools show fields
 vtools associate rare_ceu BMI --covariate SEX -m "LinRegBurden --alternative 2" -g refGene.name2 -j1 --to_db EA_RV > EA_RV.asso.res
 grep -i error *.log | tail -10
 less EA_RV.asso.res
 sort -g -k6 EA_RV.asso.res | head
 vtools associate rare_ceu BMI --covariate SEX -m "VariableThresholdsQt --alternative 2 -p 100000 --adaptive 0.0005" -g refGene.name2 -j1 --to_db EA_RV > EA_RV_VT.asso.res
 grep -i error *.log | tail -10
 less EA_RV_VT.asso.res
 sort -g -k6 EA_RV_VT.asso.res | head
 vtools select rare_ceu "refGene.name2='ABCC1'" -o chr pos ref alt CEU_mafGD10 numGD10 mut_type --header
 cd .. 
 vtools select variant --samples "RACE=0" -t YRI
 mkdir -p yri; cd yri
 vtools init yri --parent ../ --variants YRI --samples "RACE=0" --build hg19
 vtools select variant "YRI_mafGD10>=0.05" -t common_yri
 vtools select v_funct "YRI_mafGD10<0.01" -t rare_yri 
 vtools use refGene 
 vtools associate common_yri BMI --covariate SEX -m "LinRegBurden --alternative 2" -j1 --to_db YA_CV > YA_CV.asso.res
 vtools associate rare_yri BMI --covariate SEX -m "LinRegBurden --alternative 2" -g refGene.name2 -j1 --to_db YA_RV > YA_RV.asso.res
 vtools associate rare_yri BMI --covariate SEX -m "VariableThresholdsQt --alternative 2 -p 100000 --adaptive 0.0005" -g refGene.name2 -j1 --to_db YA_RV > YA_RV_VT.asso.res
 cd ..
 vtools_report meta_analysis ceu/EA_RV_VT.asso.res yri/YA_RV_VT.asso.res --beta 5 --pval 6 --se 7 -n 2 --link 1 > META_RV_VT.asso.res
 cut -f1,3 META_RV_VT.asso.res | head